Clubbers are heard gasping and screaming amid the chaos at the Florida club.
Miami Beach city manager Jimmy Morales said he had revoked Mokai Lounge’s business licence.
Police and enforcement officers have also launched an investigation after receiving multiple complaints about the incident at the South Beach venue on Thursday.
No injuries to humans or the animal have been reported.
The venue could appeal against the decision, but so far it has not commented.
The incident has sparked outrage online with many concerned about the animal’s welfare.
A petition to revoke the club’s licence was set up and pulled in more than 1,000 signatures within hours.
“People go to nightclubs to have a good time, enjoy drinks with friends, dance and party. This environment is by no means a place for ANY animal or wildlife,” the petition read.
The Miami Beach Police Department said it was “very concerned”.
It later tweeted: “Miami Beach PD detectives along with @MiamiDadePD’s animal experts have located the horse that was at Mokai. The horse has been inspected and deemed to be healthy and safe by Miami Dade PD.”
In a statement on Friday, the City of Miami Beach said it was “made aware of an incident involving possible animal cruelty and a hazard to public safety”.
Mr Morales said he was “disgusted and offended that any legitimate businessperson would think this was an appropriate action to take”.
He added: “This activity was not permitted, and as soon as we became aware, I immediately instructed staff to act swiftly in remedying this situation.”
Miami Beach mayor Dan Gelber said he “fully supported” the city manager’s decision to revoke the club’s business licence and added: “Animal cruelty is an abhorrent and vile act.”
What you mean they ain’t got no Riding Bull? I’m not going then.
But you can bring your Horse if you want?
For real?
Yeah, for real.
Ok, I’m in, I will meet you there.
https://www.youtube.com/watch?v=9OhbTECLeSQ